RNA
Part:BBa_K3385003:Design
Designed by: Daniel Bavnhřj Group: iGEM20_DTU-Denmark (2020-10-11)
gRNA backbone
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is well known and is known to work for the purpose of guiding cas9. Several cloning methods, such as USER, Gibson, and MoClo, can be used to integrate this part.
Source
Obtained as part of the CRISPR cloning vector pFC902[1].
pFC902 was a gift from Uffe Mortensen (Addgene plasmid # 106904; http://n2t.net/addgene:106904; RRID:Addgene_106904)
References
[1] Efficient Oligo nucleotide mediated CRISPR-Cas9 Gene Editing in Aspergilli. Nodvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, Mortensen UH. Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 10.1016/j.fgb.2018.01.004. 10.1016/j.fgb.2018.01.004 PubMed 29325827