RNA

Part:BBa_K3385003:Design

Designed by: Daniel Bavnhřj   Group: iGEM20_DTU-Denmark   (2020-10-11)


gRNA backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is well known and is known to work for the purpose of guiding cas9. Several cloning methods, such as USER, Gibson, and MoClo, can be used to integrate this part.

Source

Obtained as part of the CRISPR cloning vector pFC902[1].

pFC902 was a gift from Uffe Mortensen (Addgene plasmid # 106904; http://n2t.net/addgene:106904; RRID:Addgene_106904)

References

[1] Efficient Oligo nucleotide mediated CRISPR-Cas9 Gene Editing in Aspergilli. Nodvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, Mortensen UH. Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 10.1016/j.fgb.2018.01.004. 10.1016/j.fgb.2018.01.004 PubMed 29325827